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Expression of dicysteine mutants in Xenopus oocyte membranes.
figure
posted on 2013-02-20, 08:41 authored by Mike Mueckler, Carol MakepeaceStage 5 Xenopus oocytes were injected with water (Sham) or with 50 ng of mRNA encoding the parental reporter construct (C-Tev) or the indicated dicysteine mutant. Two days post injection total oocyte membranes were prepared and subjected to immunoblot analysis using a rabbit polyclonal ab raised against a peptide corresponding to the C-terminal 15 residues of human Glut1.