Expression evaluation of the DR-related genes during HLR.
Seedlings of Samsun nn and Samsun NN were inoculated with in vitro transcripts of wt TMV (Red), TMVFN20 (Yellow) and TMVSC1754 (Green) as mentioned in Experimental Procedures. Infection buffer (Blue) was used as negative control. Inoculated seedlings were incubated at 25°C and 33°C respectively and sampled for real-time PCR assay at 0, 2, 4, 6 dpi. Data are shown as the mean of at least two biologically repeated experiments, and the error bar is the standard error (SE). Expression values of each gene are presented as the percentage of the reference gene ubiquitin. At 0 dpi, average expression values of the genes in Samsun plants at 25°C are 0.45±0.18 for PR-1a (A), 3.16±0.68 for HMGR (B), 0.05±0.01 for HIN1 (C), and 0.03±0.00 for N (D) in terms of mean ± SE percent of ubiquitin, respectively. Signals of each gene at different time points (2, 4, 6 dpi) were compared to that at the initial time point (0 dpi) by t test, *p<0.01.