Experimental design of (a) the F344 rat study and (b) the nude (nu/nu) mouse- human colon tumor xenograft study.

Numbers in boxes depict the time (in weeks) after the animals arrived at our housing facility. In both studies, a one-week acclimatization period was maintained before specific interventions. Diets were based on AIN-93G semi-synthetic formulation and the four experimental diets differed from one another in the level of acrylamide added: 0 (control), 0.5, 1.0 and 2.0 mg/kg diet. All animals remained on the respective experimental diets until necropsy.