Existence of a persistent, mislocated X-like-zone in AdKO adrenals.

The X-zone 20α-HSD marker (in red) and the zona fasciculata Akr1b7 marker (green, right column) were co-immunodetected. The two colours are merged in the right column with the Hoechst nuclei marker (blue). (A) Adrenal section of a 5-month-old nulliparous WT female. The unlabelled zone separating the X-zone (red) and the zona fasciculata (green) is indicated by a star (*). (B) Adrenal section of a 5-month-old nulliparous AdKO female. (C) Adrenal section of a 10-month-old parous WT female. (D) Adrenal section of a 10-month-old parous AdKO female. (E) Adrenal section of a 18-month-old parous AdKO female. M, Medulla; F, zona fasciculata; G, zona glomerulosa; X, X-zone; XL, X-like-zone; Scale bars, 50 µm.