Example of results of the semi-quantitative SLM drop assay showing various categories of mutator phenotype.

Cell suspensions were serially diluted and spotted onto selection plate (with canavanine or 5′-FOA) and onto dilution control plate as described in Materials and Methods. WT – SLM level in parental strain, M - increased SLM phenotype, HM - high SLM phenotype, ρ - increased SLM due to respiratory incompetence in WT ρ strain, Rρ – resistance to selection conditions acquired along with the loss of respiratory competence, M/GD - high SLM phenotype accompanied by decreased survival rate, seen also without selection, R - full resistance to selection conditions.

Categories

License

CC BY 4.0