Figure 8.tif (999.16 kB)
Evaluation of the interaction between Foxe3- (A) and Mip- (B) bicoid sites and proteins in wild-type and –miak mice by electrophoretic mobility assay (EMSA).
dataset
posted on 2014-10-27, 17:08 authored by Kenta Wada, Yoshibumi Matsushima, Tomoki Tada, Sayaka Hasegawa, Yo Obara, Yasuhiro Yoshizawa, Gou Takahashi, Hiroshi Hiai, Midori Shimanuki, Sari Suzuki, Junichi Saitou, Naoki Yamamoto, Masumi Ichikawa, Kei Watanabe, Yoshiaki KikkawaEMSA performed with Foxe3- [16] and Mip- [21] bicoid oligonucleotides (oligo probe) and nuclear extracts (NE) from wild-type and miak eyes at E17.5. Although the formation of the specific EMSA complex occurred by combining oligo probes and NEs from wild-type and miak mice, the binding ability was increased with miak-NE and both Foxe3- and Mip-bicoid oligo probes. The binding ability of both oligo probes was inhibited by 10-fold excess unlabeled competitive probes (competitor).
