Etk and Wzc are not essential for Etp phosphorylation.

<p>EtpD119A was expressed in different EPEC strains with deletions in the <i>etp</i> gene and the kinase genes <i>etk</i> and <i>wzc</i>. Proteins were extracted and the amount and phosphorylation state of Etp were tested by Western blot using anti-6His and anti-PY antibodies, respectively. The presence of intact genes is indicated above the lanes.</p>




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