Enhancement of the rRNA transcription rate does not increase recombinant protein synthesis.
a. qRT-PCR of 45S rRNA levels in HEK293T and HeLa following expression of increasing amounts of UBF (pCMV-UBF). rRNA levels were normalized to GAPDH mRNA quantities. Data are from two independent experiments. b. SEAP expression of HEK293T and HeLa cells co-transfected with pCAG-SEAP and either pCMV-UBF or pCMV-TAP-tag (control). Data are from two independent experiments. c. Polysome profile of HEK293T cells transfected with either pCMV-UBF or pCMV-TAP-tag (control). The data show one representative experiment out of two independent experiments.