Enhanced robustness in adult emergence of selected population persists under semi-natural (SN).
(A) Circadian waveform of emergence of selected (PP) and control (CP) flies under laboratory (LAB). Percentage of flies that emerged in 2 h bins over five successive cycles is plotted along y-axis and External Time (in h) along x-axis. The square wave indicated above the figure represents 12∶12 h light/dark (LD) cycles. (B) Circadian waveform of emergence under SN. Percentage of flies that emerged in 2 h bins over five successive cycles are plotted along y-axis and External Time in h along x-axis. Upper panels of 1B show daily profiles of light (lux), temperature (°C) and humidity (%RH) for the entire duration of the experiment. Under LAB, greater percentage of PP flies emerged during the peak which persisted under SN. For this assay, ten vials for each of the four replicate populations were used under each environmental condition. The mean values of four replicates and variance in terms of 95% comparison interval (95%CI) were used to draw emergence profiles and error bars.