Enhanced cytokine recall responses of cblb-silenced CD8+ T cell tumor infiltrates.

Tumor infiltrating CD8+ T cells either nucleofected with control siRNA or cblb siRNA were ex vivo stimulated with unloaded or SIINFEKL loaded DCs and (A) IL-2 as well as (B) IFNγ secretion was measured by BioPlex technology. Means ± SEM of four independent experiments are shown. (A) p = 0.03 (SIINFEKL loaded DC+CD8+cblb siRNA versus SIINFEKL loaded DC+CD8+ control siRNA), (B) p = 0.05 (SIINFEKL loaded DC+CD8+cblb siRNA versus SIINFEKL loaded DC+CD8+ control siRNA).