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Engraftment of wild-type and MyoD−/− myoblasts after myocardial injection into infarcted heart.

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posted on 2013-02-20, 01:53 authored by Yasuhiro Nakamura, Yoko Asakura, Bryan A. Piras, Hiroyuki Hirai, Christopher T. Tastad, Mayank Verma, Amanda J. Christ, Jianyi Zhang, Takanori Yamazaki, Minoru Yoshiyama, Atsushi Asakura

(A) These panels show MI induced by left coronary artery ligation. Wild-type and MyoD−/− myoblasts were directly injected into the peri-infarct regions of LV. After 1 week, X-gal staining of whole heart indicated that more MyoD−/− myoblasts engrafted than wild-type myoblasts (arrows). Arrowheads indicate left coronary artery ligation points. X-gal staining of cross sections indicated that more MyoD−/− myoblasts than wild-type myoblasts engrafted in both injured and uninjured areas of the heart. Arrows indicate engrafted lacZ+ wild-type and MyoD−/− myoblasts. Scale bars = 1 mm. (B) Engraftment of injected cells in the infarcted heart. More MyoD−/− myoblasts than wild-type myoblasts engraft in the infarcted heart 3 day to 4 weeks post-transplantation. Y-axis indicates the survival rates of engrafted cells after an injection of 1×106 wild-type or MyoD−/− myoblasts. (C) By day 3, anti-activated caspase-3 (Casp-3) and anti-phospho-histone H3 (p-His-H3) antibody staining demonstrated that lacZ+ wild-type and MyoD−/− myoblasts proliferate or undergo apoptotic cell death (n = 4). Nuclei were counter-stained with DAPI (blue). Scale bar = 50 µm. (D) Comparison of the relative numbers of lacZ+/activated caspase-3+ or lacZ+/phospho-histone H3+ cells for wild-type and MyoD−/− myoblasts 3 days after transplantation.

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