Endothelial cell activation and stress in the liver and lungs.

Mice (4–5/group) were inoculated with O. tsutsugamushi Karp stain, as described in Fig. 1. Liver and lung tissues were collected at 0, 2, 6, and 10 dpi and analyzed via qRT-PCR for gene expression. (A, C) Data are shown as mean ± SEM in each group and are presented as “relative mRNA level” (after normalization to the housekeeping genes). (B, D) The Ang-2/Ang-1 ratios of individual samples were calculated based on the corresponding data in A and C. *, p<0.05; **, p<0.01.