Public Library of Science
Browse
Figure_1.tif (1.53 MB)

Efficient selection of CD4+ T lymphocytes in NODmini and NODβTg mice.

Download (0 kB)
figure
posted on 2014-11-07, 03:44 authored by Joanna Kern, Robert Drutel, Silvia Leanhart, Marek Bogacz, Rafal Pacholczyk

Lymphocytes isolated from thymii (A) and lymph nodes (B) of indicated mice were stained with monoclonal antibodies and analyzed by flow cytometry. Numbers in quadrants are representative percentages of at least six mice (6 week old) per group. The numbers of thymocytes and lymphocytes ± SD recovered from NODmini, NODβTg and NOD mice were: from thymii 78.5±16.7×106, 72.05±13.8×106 and 70.1±14.0×106, and from lymph nodes (axillary, brachial and inguinal) 17.2±5.8×106, 14.1±2.5×106, and 13.2±3.0×106, respectively. (C) Percentages (top) and total numbers (bottom) of CD4+Foxp3+ T cells in peripheral lymph nodes of 6 week old mice; each circle represents individual mouse. (D) Expression of mRNA of TCRVα genes in sorted CD4+ T cells isolated from NOD and NODβTg mice. Analysis was done by RT-PCR using primers specific to indicated Vα segments and Cα region. (E) Comparison of CD4+Foxp3+ T (Treg) cells from thymii and peripheral lymph nodes of transgenic and wild type B6 and NOD mice. Mean percentage and SD of six young mice per group are shown.

History

Usage metrics

    PLOS ONE

    Categories

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC