Effects of chloroquine on zinc ion uptake.
A. A2780 cells were plated in a 96-well plate and treated with chloroquine (ChQ) alone or in combination with ZnCl2 at the indicated concentrations for 1 hour. The FluoZin-3 (1 µM) probe was added and the fluorescent signal was measured by excitation at 490/20 nm and emission at 528/38 nm. Data (n = 3, bars, SEM) are presented as a percentage of the highest fluorescence level detected. *, p<0.01, compared to cells without ChQ treatment, using One-way ANOVA followed by Bonferroni analysis. B. A2780 cells were plated in a 12-well plate and treated with ChQ and ZnCl2 at indicated concentrations for 1 hour. After addition of the FluoZin-3 probe, cells were examined under a fluorescent microscope by excitation at 490/20 nm and emission at 528/38 nm. Shown are representative images from three individual experiments.
