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Effects of GA and 3-OHGA on neurons.

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posted on 2013-02-19, 17:14 authored by Paris Jafari, Olivier Braissant, Petra Zavadakova, Hugues Henry, Luisa Bonafé, Diana Ballhausen

(Left panel) Immunohistochemical staining for phosphorylated medium weight neurofilament (p-NFM) on cryosections of cultures derived from protocol A (DIV 8) and protocol B (DIV 14). Scale bar: 100 µm. (Right panel) Representative western blots with data quantification of whole-cell lysates for p-NFM for protocol A (DIV 8, above) and protocol B (DIV 14, below). Actin was used as a loading control. The quantifications of p-NFM are expressed as percentage of respective controls. The values represent the mean ± SD from 3 replicates taken from 2 independent experiments.

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