Effects of CXCR4 antagonist AMD3100 on colonic damage and disease activity index in mice.
Colonic morphology from control groups (A), colitis group (B), and AMD3100 group (C) was examined in a blind-fashion. Colonic TNF-α (D), IL-6 (E), and IFN-γ (F) were evaluated by enzyme-linked immunosorbent assay. The histological score (G) was also determined. The weight loss (H), the fecal characteristics and fecal blood were monitored each day, and the disease activity index (I) was determined at day 4 and 8. The colonic epithelial barrier (J) was assessed using mucosal-to-serosal clearance of permeability probe FD4 in everted gut sacs. Eight mice were studied in each experimental group. Results are mean ± SEM. *P<0.05, colitis group vs control group; ** P<0.05, AMD3100 group vs colitis group. Original magnification, 200×.