Effects of CHF5633 on IL10 mRNA and protein expression in LPS-activated adult human monocytes.

Monocytes were stimulated with 100ng/ml LPS and subsequently exposed to 100μg/ml CHF5633 or 100μg/ml Curosurf®(A, B) or 100μg/ml POPG, 100μg/ml PLs, 100μg/ml PLs+SP-B and 100μg/ml PLs+SP-C, respectively (C). IL-10 mRNA expression was assessed at 4h (A) and 14h (B) qPCR. IL-10 protein expression was analyzed by means of flow cytometry at 14h (C). Results are expressed as mean ± SD (*p < 0.05, **p < 0.01).