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Effect on yeast intake and CDM contractions by Gal4-directed dTrpA1-mediated activation of neuronal networks.

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posted on 2014-06-24, 03:22 authored by Andreas Schoofs, Sebastian Hückesfeld, Philipp Schlegel, Anton Miroschnikow, Marc Peters, Malou Zeymer, Roland Spieß, Ann-Shyn Chiang, Michael J. Pankratz

(A) Experimental setup: yeast intake of larvae (% of body stained) was determined after 20 min of dTrpA1 activation (upper picture). All tested Gal4-lines showed a decreased relative change in yeast intake except 5-HT (TRH-Gal4) line, which showed an increase (lower panel; Mann-Whitney Rank Sum Test: ***p≤0.001). (B) CDM contractions were tracked by measuring the length difference of pharyngeal lumen (Δd) at 32°C relative to the maximal contractions at 18°C (upper picture). Tracking of the CDM contractions correspond to deduced muscle activity based on the AN recordings (lower left panel). CDM contractions were quantified as relative change in contractions/min (lower right panel). Significance was tested by Mann-Whitney Rank Sum Test (*p≤0.05, ***p≤0.001). 5-HT, serotonin; ACh, acetylcholine; DA, dopamine; Glu, glutamate; Hug, hugin neuropeptide.

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