Effect on yeast intake and CDM contractions by Gal4-directed dTrpA1-mediated activation of neuronal networks.
(A) Experimental setup: yeast intake of larvae (% of body stained) was determined after 20 min of dTrpA1 activation (upper picture). All tested Gal4-lines showed a decreased relative change in yeast intake except 5-HT (TRH-Gal4) line, which showed an increase (lower panel; Mann-Whitney Rank Sum Test: ***p≤0.001). (B) CDM contractions were tracked by measuring the length difference of pharyngeal lumen (Δd) at 32°C relative to the maximal contractions at 18°C (upper picture). Tracking of the CDM contractions correspond to deduced muscle activity based on the AN recordings (lower left panel). CDM contractions were quantified as relative change in contractions/min (lower right panel). Significance was tested by Mann-Whitney Rank Sum Test (*p≤0.05, ***p≤0.001). 5-HT, serotonin; ACh, acetylcholine; DA, dopamine; Glu, glutamate; Hug, hugin neuropeptide.