Effect of silencing Cx43 on the expression of HIF-1α in astrocytes.

Astrocytes were transfected with NT-siRNA or with Cx43-siRNA. At the indicated times the expression of HIF-1α and Cx43 was analysed by Western blot. A) Representative Western blot of HIF-1α, Cx43 and GAPDH showing that the decrease in Cx43 expression was concomitant with HIF-1α up-regulation. B) HIF-1α quantification. C) Cx43 quantification. The results are expressed as percentages of the level found in the NT-siRNA condition at time 0. ***p<0.001 versus the corresponding NT-siRNA values.