Effect of normalization strategy on statistical parameters calculated for Nppb and Vcam1 expression using combinations of multiple reference genes.

Two-tailed Mann-Whitney U test was used for calculating p-values. The statistical power was calculated for a Student's t-test using statistical parameters of log₂ transformed expression data. Sample size (n) is number per group needed to obtain a power of at least 0.8. Nppb: group 2 vs. group 3; Vcam1: group 1 vs. group 3.