Effect of leptin receptor function on the survival, the degree of lung injury and inflammation during influenza A infection.

db/db and C57BL/6 mice were inoculated with influenza A virus (A/WSN/33 [H1N1]) (a) 1500 pfu/mouse or (b) 500 pfu/mouse and mortality was subsequently assessed on a daily basis. Percentage of weight loss was also followed daily for the mice infected with (c) 1500 pfu/mouse or (d) 500 pfu/mouse. Four days after infection with influenza A virus 1500 pfu/mouse, db/db and C57BL/6 mice underwent a bronchoalveolar lavage for (e) assessment of cell count, (f) total protein, (g,h) inflammatory cytokines, (i) flow cytometry, and (j) the assessment of lung pathology (Hematoxylin and Eosin staining). * p<0.05 compared to PBS control. ** p<0.05 db/db compared to wild-type mice.