Effect of endoglin haploinsufficiency on extracellular matrix formation.

(A) Synthesis of collagen Iα and fibronectin by Eng+/+ and Eng+/− MDF were analyzed by RT-PCR. GAPDH was used as housekeeping (B) Synthesis of collagen Iα and fibronectin were also evaluated by western blot in total protein extracts from Eng+/+ and Eng+/− MDF using Tubulin as loading control. A representative blot from three independent experiments is shown.