Effect of TGFα on KGN cell viability and DNA synthesis.

A) KGN cells were treated with or without TGFα for 48 hours and cell viability was detected with MTT assay. Bars represent means ± SEM. Bars with different letters are significantly different (P<0.05) from each other. B) Effect of TGFα on KGN cell DNA synthesis. KGN cells were treated with or without TGFα for 36 hours and DNA synthesis was detected by BrdU incorporation. A representative image for BrdU immunofluorescence is shown for both control and TGFα treated groups. Magnification, 200×. Bars represent means ± SEM. “a”, significantly different (P<0.05) from the control group.