Effect of H₂S on the expression of SIRT-1 in PC12 cells.

A, PC12 cells were treated with different concentrations of NaHS (100, 200 and 400 µmol/L) for 24 h. B, PC12 cells pretreated with NaHS at a concentration of 200 µM for 30 min prior to 24-h exposure of formaldehyde (FA, 120 µM). The levels of SIRT-1 expression in PC12 cells were detected by Western blot using anti-SIRT-1 antibody. In all blots, staining for β-actin was used as a loading control. Values are the mean ± S.E.M. of three independent experiments. *P<0.05; **P<0.01; ***P<0.001, vs control group; ^#P<0.05, vs FA-treated alone group.