Effect of Ber on caspase activities, Bcl-2 protein level, cytoplasmic cytochrome c (Cyt c) and mitochondrial Bax contents in DOX-treated cardiomyocytes.

(A, B and C) Cardiomyocytes were treated with DOX (1.0 µM) in the absence or presence of Ber (1.0 µM) for 12 h, caspase-3 (n = 4), caspase-8 (n = 4) and caspase-9 (n = 6) activity were analyzed by flow cytometry. (D, E and F) Cardiomyocytes were treated with DOX (1.0 µM) in the absence or presence of Ber (1.0 µM) for 6 h, levels of cytoplasmic Cyt c, mitochondrial Bax and Bcl-2 (whole cell homogenate) proteins were determined by Western blotting (n = 4). *P<0.05, **P<0.01 compared with control group. ^#P<0.05, ^{# #}P<0.01 compared with DOX group.