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Effect of ABCG2 overexpression on the expression of SLC transporter genes and MPP, glucose and glutamine transport.
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posted on 2015-11-04, 06:20 authored by Maricla Galetti, Pier Giorgio Petronini, Claudia Fumarola, Daniele Cretella, Silvia La Monica, Mara Bonelli, Andrea Cavazzoni, Francesca Saccani, Cristina Caffarra, Roberta Andreoli, Antonio Mutti, Marcello Tiseo, Andrea Ardizzoni, Roberta R. AlfieriDown-regulation of SLC transporter expression (SLC22A1, SLC22A3, SLC2A1, SLC38A2, SLC3A2, SLC7A5, and SLC7A6) in HEK293/R2 cells versus HEK293 was evaluated by PCR drug transporter array (A). Fold decrease expression was determined by the ΔΔCT method normalized to five housekeeping genes. Values given are the means (± SD) of three independent experiments (*P<0.05; **P<0.01). Initial velocity (5 min) of [3H]MPP (B), [3H]2-deoxy-D-glucose (C) and [3H]Glutamine (D) uptake was determined and values given are the means (± SD) of three independent determinations (*P<0.05; **P<0.01).
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ABCG 2 plasma membrane levelsABCG 2efflux transporter ABCG 2uptakeintracellular gefitinib contenttyrosine kinase inhibitorscell lung cancerintracellular gefitinib accumulationABCG 2 overexpressioncancer chemotherapeutic agentsmultidrug resistance proteinEGFR Tyrosine Kinase Inhibitordrug transport systemsmppNSCLC cell lineslung cancer cellsABCG 2 activity
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