Down-regulation of <i>RPH3AL</i> mRNA and protein in breast cancers.

<p>Analyses of mRNA and protein expression of <i>RPH3AL</i><b>(A)</b> mRNA levels of <i>RPH3AL</i> down-regulated in breast cancers relative to their matching normal tissues. <b>(B)</b> Immunohistochemical staining of RPH3AL in breast cancer tissues. RPH3AL expression was seen predominantly in the cytoplasm of <b>(i)</b> lobular intraepithelial neoplasia (LIN, orange arrow) and adjacent normal cells (green arrow) (X100) <b>(ii)</b> ductal carcinoma in situ (DCIS, red arrow) and adjacent normal cells (green arrow) (X100) <b>(iii)</b> DCIS (red arrow) and invasive carcinoma (dark red) (X200). LIN and DCIS exhibit lower expression of RPH3AL relative to adjacent normal cells. Mild nuclear staining was also seen in a few cells of normal, LIN, DCIS, and invasive carcinoma.</p>