Double staining of a larval wing imaginal disk and pupal wing tissue.

2015-06-24T04:18:44Z (GMT) by Yoshikazu Ohno Joji M. Otaki

The tissue was stained with SYBR Green I for nuclei and MitoTacker Orange for mitochondria. Scale bars indicate 10 μm. (a) Larval wing imaginal disk. A portion is enlarged in the right panel. An optical vertical cross-section image shown at the bottom was made along a blue broken line. Thickness of a cell was approximately 2.9 μm. The image was constructed by stacking 28 images down to 5.5 μm in depth with 0.2 μm intervals. (b) Pupal wing tissue. The pupal head is positioned to the left. A portion is enlarged in the right panel. An optical vertical cross-section image shown at the bottom was made along a blue broken line. Thickness of a cell was more than 11.4 μm (the deepest portion not detected). The image was constructed by stacking 58 images with 0.2 μm intervals.