Disruption and complement of spmABC genes.

A. Growth curve of S16 (▪) and the spmABC genes disrupted mutant S16dspm (□) with nicotine as the sole carbon and nitrogen source. B. HPLC analysis of nicotine degradation (solid) and SP formation (open) by resting cells of P. putida S16 (square) and P. putida S16dspm (triangle). Symbol definition: nicotine degradation by S16 (▪solid square), nicotine degradation by S16dspm (▴solid triangle), SP formation by S16 (□open square), SP formation by S16dspm (▵open triangle). C. Growth curves of S16 (▪), S16dspm with plasmid pME6032 (○), and S16dspm with complementary plasmid pME6032-spm100 were analyzed by Bioscreen C with nicotine as the sole carbon and nitrogen source. D. HPLC analysis of SP degradation by resting cells of P. putida S16 (▪), P. putida S16dspm (pME6032) (•), and P. putida S16dspm (pME6032-spm100) (▴).

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