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Differential specificity of Dictyostelium Sfp (DiSfp) towards mycobacterial PKS2 and kinetic analysis.

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posted on 2011-09-12, 01:49 authored by Divya R. Nair, Ratna Ghosh, Alzu Manocha, Debasisa Mohanty, Shweta Saran, Rajesh S. Gokhale

Gel-binding assays were set up with DiAcpS and DiSfp, taking [1-14C] acetyl-CoA (A-CoA), [1-14C] hexanoyl-CoA (H-CoA) and [1-14C] lauroyl-CoA (L-CoA) as co-substrates. A, Panels show the autoradiogram of SDS-PAGE in which mycobacterial PKS2 was labeled by different acyl CoA substrates and PPTases as indicated. B, Gel-based kinetic analysis of DiSfp with respect to acetyl CoA. Concentration of acetyl CoA was varied from 5 µM to 45 µM.

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