Developmental effects of auxin insensitivity.

<p><b>(A)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> lines suffer from severe dwarfism compared to <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL</i> plants. <b>(B)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> line showing a lack of organized patterning, dorsiventral polarity and a limited degree of differentiation. Scales (arrowhead), air pores (asterisk) and rhizoids (r) are visible in random places and growth is severely affected. <b>(C)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> representative line showing a degree of differentiation similar to the prothallus stage of wild-type development. <b>(D)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> showing single cell layer prothalli-like tisssue. <b>(E)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF2</i></sup> representative line. <b>(F)</b> Wild-type sporeling (10 days) transitioning into a single cell layered prothallus (14 days) and fully established multiple prothalli 18 days after plating. <b>(G)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>BDL</i></sup> line lacking growth, differentiation and dorsiventrality, but still capable of producing rhizoids. <b>(H)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF3</i></sup> lines differentiate gemmae cups and a dorsiventral polarity with dorsal air chambers and ventral rhizoids. The thallus has an apparent hyponastic curvature. <b>(I, J)</b> Multiple independent Hyg<sup>Res</sup> sporelings (transformed with an empty binary vector) grown on media containing 10μg/ml Hyg and mock (<b>I</b>) or 10μM 2,4-D (<b>J</b>) for 14 days. <b>(K, L)</b> Multiple independent <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>K</b>) or <sub><i>pro</i></sub><i>MpTPL</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>L</b>) lines grown on 10μM 2,4-D for 14 days. Scale bars in C, E, I, J, K and L = 1mm; A = 1cm; B = 430μm; D = 130μm; F = 30μm; G = 600μm; H = 860μm.</p>