Determination of hepatic steatosis (hepatic histology) in control mice and mice with high fat diet-induced obesity, administered or not B. uniformis CECT 7771.

SD: standard diet group (control) (n = 6); SD+B: standard diet group receiving a daily dose of 5.0×108 CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6); HFD: high fat diet group (n = 6); HFD+B: high fat diet group receiving a daily dose of 5.0×108 CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6).The fat vacuoles were measured in 100 hepatocytes of two liver tissue sections per mouse and scored for the severity of steatosis according to the following criteria: For grade-0 steatosis, no fatty hepatocytes; grade-1 steatosis, fat occupying less than 30% of the hepatocyte; grade-2 steatosis, fat occupying less than 30 to 60% of the hepatocyte; grade-3 steatosis, fat occupying more than 60% of the hepatocyte. Photomicrographs 20X of representative HE-stained slides are shown. (B) SD group, (C) SD+P group, (D) HFD group and (F) HFD+P group. Data are expressed as means ± SD and statistically significant differences are established at P<0.05.