Detection of chikungunya in mouse muscle tissues using Immunofluorescence assay after chikungunya infection and siRNA treatment.

C57BL/6 mice were infected with CHIKV i.v. (1×106 PFU CHKV; 100 µl of 107pfu/ml). PBS, ncsiRNA, E2 siRNA and ns1 siRNA injected mice showed absence of chikungunya antigen at 4th and 7th days p.i. (A, B, C, D, E and F). CHIKV infected muscles showed presence of chikungunya antigen (G and H), ncsiRNA treated CHIKV infected muscles showed presence of chikungunya antigen (I and J) whereas siRNA treated CHIKV infected mice muscle tissues showed the faint staining of chikungunya antigen (K, L, M, N, O and P) (Magnification ×200).