Detection of Mycobacterium avium spp paratuberculosis’ genomic marker in clinical samples with hMRS and culture-based PCR.

(a) Blood samples from a second population (Cohort 2, n  =  26) were screened for the presence of the MAP IS900 genomic element with hMRS, direct nPCR and culture-based nPCR after 12-week cultivation. The receiver operating characteristic (ROC) curve indicated that the hMRS, can better differentiate between symptomatic (Chrohn’s) and asymptomatic (healthy) states than the two gold standard methods. (b) Two-proportion z-test confirmed hMRS as a better clinical diagnostic utility than the PCR-based methods.