Derivatives of dose-response curves.
A. From each dose-response curve, four different derivatives can be calculated to assess TLR-responsiveness: hillslope (maximum steepness of the curve), EC50 (ligand concentration resulting in half-maximum activation), area under the curve (AUC) and the delta (difference between minimum and maximum expression levels). B. Representative selection of CD11b and L-selectin response curves from individual patients showing high inter-individual variability after LPS stimulation.