Depletion of Treg cells induces balanced Th1/Th2/Th17 immunity in both stages of infection.

(A) Quantification of activated/memory and naïve CD4⁺ and CD8⁺ T lymphocytes in lungs from mice treated with anti-CD25 or control IgG and analyzed after 2 (left) and 10 (right) weeks of infection. (B) mRNA relative expression of Tbet, GATA3, RORγC, Foxp3, PD-L1, CCR5, CCR6 and Granzyme B in lung cells of mice treated with anti-CD25 or control IgG, after 2 (top) and 10 (bottom) weeks of infection. (C) Cytokine quantitation by ELISA in lung homogenates from mice treated with anti-CD25 or control IgG. Bars show mean ± SD from at least five mice per group and are representative of three independent experiments (*p< 0.05, **p < 0.005, ***p <0.001).