Dendritic cells are infected by Bartonella.
(A) Effector translocation by Bhe into mouse bone marrow-derived dendritic cells (BMDCs). Balb/c mouse BMDCs were infected with corresponding MOIs and strains. “Effector”, Bla-BID, translocation efficiency was assessed as the % of infected cells that converted CCF2-AM blue emission into green detected by Leica DM-IRBE inverted fluorescence microscope. The bars represent the mean of triplicate samples +/− SD. Data from one representative experiment (n = 2) are presented. (B) Migration of BMDCs is inhibited in a trans-well assay by Bhe ΔbepDEF infection. BMDCs were pre-infected with MOI = 50 of the indicated bacterial strains. Infected cells were embedded in collagen and mounted in a trans-well migration system that was prior seeded with a confluent monolayer of iLECs (immortalized lymphatic endothelial cells). BMDCs that migrated though the iLECs were quantified after 24 h. The data normalized to uninfected condition. The bars represent the mean of triplicate samples +/− SD. Statistical significance was determined using Student's t-test. P<0.05 was considered statistically significant. Data from one representative experiment (n = 3) are presented.