Daily rhythms of blood glucose and some metabolic parameters related to cholesterol homeostasis in mice fed time-restrictively.

Young adult male C57BL/6J mice were first entrained to a 12∶12 LD photoperiodic cycle for two weeks. Then mice were fed time-restrictively for seven consecutive days as denoted in Figure 1. Mice were sacrificed by cervical dislocation at the indicated ZT and whole blood samples were collected. Total cholesterol (A), HDL cholesterol (B), plasma triglyceride (C), blood glucose (D) levels were determined by specific kits obtained from CallegariTM. All data are expressed as mean ± S.E.M. (n = 4–8). Statistical analyses are summarized in Table 2.