Figure_2.tif (2.46 MB)
DNR induced autophagy in K562 cells.
figure
posted on 2011-12-02, 02:18 authored by Weidong Han, Jie Sun, Lifeng Feng, KaiFeng Wang, Da Li, Qin Pan, Yan Chen, Wei Jin, Xian Wang, Hongming Pan, Hongchuan JinA. K562 cells were incubated with varying concentrations of DNR for 24 hours or incubated with 1.25 µg/ml DNR for appropriate intervals. The switch of LC3-I to LC3-II was detected by immunoblotting. B. Immunoblotting for Beclin-1 using lysates from K562 treated with 1.25 µg/ml DNR for appropriate intervals. C. MDC staining of K562 treated with DNR. Cells treated with 1.25 µg/ml DNR in the presence or absence of 5 µM CQ for 24 hours were stained with MDC. D. K562 cells were treated with 1.25 µg/ml DNR for 24 hours, and stained by indrect immunofluorescence as described in Marterial & Methods. The distribution of endogenous LC3 was monitored at confocal microscope.