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DIDS does not effect intracellular expression of pro- or active MMP-2 or -9 isoforms.

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posted on 2012-08-24, 02:14 authored by Matthew E. Pamenter, Julie Ryu, Serena T. Hua, Guy A. Perkins, Vincent L. Mendiola, Xiang Q. Gu, Mark H. Ellisman, Gabriel G. Haddad

IS and STS treatment increased intracellular expression of proMMP-2 and -9 isoforms, as well as the active form of MMP-2. DIDS did not effect these changes. (A) Sample Western blots of MMP-2 and -9 protein expression. Latent and active isoforms of MMPs were detected at 66 and 62 kDA (MMP-2) and 96 and 88 kDA (MMP-9), respectively. (B&C) Summaries of neuronal latent and active MMP-2 (B) and MMP-9 (C) protein isoform expression from analysis of cellular fractions normalized to the cellular expression of α-actin on the same blot. Data are presented as fold-change relative to untreated controls. Data are mean ±SEM from 3-5 separate 6-hr experiments. Asterisks (*) indicate significant difference from normoxic controls (p<0.05). Treatments as per Fig. 1 caption.

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