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Cytokine production by mouse macrophage cell line RAW264.7 exposed to BtCDC272 grown in different conditions and at different M.O.I.

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posted on 2014-03-26, 03:02 authored by Clelia Peano, Fabrizio Chiaramonte, Sara Motta, Alessandro Pietrelli, Sebastien Jaillon, Elio Rossi, Clarissa Consolandi, Olivia L. Champion, Stephen L. Michell, Luca Freddi, Luigi Falciola, Fabrizio Basilico, Cecilia Garlanda, Pierluigi Mauri, Gianluca De Bellis, Paolo Landini

Confluent monolayers of cells were incubated with bacteria cultured at the indicated conditions. Bacteria were added at MOI of 0.005, 0.05, 0.5 or 5 for 4 hours to confluent monolayers of RAW264.7 cells. LPS (100 ng/ml from E. coli Serotype 055:B5; Sigma-Aldrich) was used as positive control of macrophage activation. Murine CXCL2, TNF-α and IL-6 levels were measured in cell supernatants by ELISA Results are mean± SEM of two independent experiments.

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