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Cryopreservation interferes with the allogeneic lymphoproliferative response.

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posted on 2013-07-31, 01:45 authored by Guilherme Ferreira Silveira, Pryscilla Fanini Wowk, Anália Maria Breckenfeld Machado, Claudia Nunes Duarte dos Santos, Juliano Bordignon

(A) Representative histogram of autologous and allogeneic lymphocyte proliferation of one donor (frh-imdDCs (black), crp-imdDCs (blue) and Lyn-stained lymphocytes without DC stimulation (red)). (B) Frh- (circles) and crp-imdDCs (squares) were co-cultured with autologous (negative control) or allogeneic lymphocytes stained with CellTracker™ Green CMFDA in a 1∶10 ratio of imdDCs:lymphocytes. After five days of co-culture lymphocytes proliferation was analyzed by flow cytometry. Data was analyzed by Student’s t test and the values shown are the means ± SDs of six individual donors. ***p≤0.001. frh: freshly obtained, crp: cryopreserved, imdDCs: immature monocyte derived dendritic cells, Lyn: stained lymphocytes without DC stimulation, Aut lyn: autologous lymphocytes, All lyn: allogeneic lymphocytes.

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