Confocal microscopic images of Streptococcus pneumoniae D39 biofilms grown with and without pyrimidinedione.

(A & B) Representative confocal images of a control sample. The biofilms in the control sample were thick with an organized 3-dimesional structure. (C & D) Representative confocal images of biofilms grown with 7 μM/ml pyrimidinedione. The pyrimidinedione-grown biofilms were thin and disorganized, with clumps of cells attached to the bottom of plate.