Confirmation of the Hits by flow cytometry on ALP.
A) Evaluation of the osteogenic potential of several treatment conditions by flow cytometry on hMSCS after 4 days treatment. Data is expressed as percentage of ALP positive cells. At least 10,000 cells were measured for each condition and experiments were performed in triplicate. Error bars represent standard deviation. Statistical analysis was performed using (one-way ANOVA and Tukey post-test with a significance level of 0.05. Asterisks represent * P<0.05, ** P<0.01 and *** P<0.001. BM (basic medium); OM (Osteogenic Medium); DEX (Dexamethasone). B) Number of donors in which the respective compound had a positive effect on ALP induction both in basic or osteogenic medium.