Concentration of IFN-γ-secreting cells in the mice immunized with pRC/CMV-VP1 with or without oral administration of LASW1.

ELISpot assay was used to determine the number of cells secreting IFN-γ in the spleen. A single-cell suspension of lymphocytes was prepared and stimulated in culture with 146S antigen, PHA as positive control, and BSA as an irrelevant antigen. Results represent mean ± SD of number of IFN-γ producing cells of 3 male mice per group in triplicate wells.