Computational protocol used to detect local similarities between ATP-binding sites in Pim-1 kinase and Synapsin I.

a) The ATP-binding site in Pim-1 kinase (occupied by the ligand staurosporine) is compared with SiteAlign [41] (step a) to 6,415 binding sites stored in the sc-PDB database . Among top scoring entries (step b), synapsin I is the only protein not belonging to the protein kinase target family (step c) and present in numerous copies (step d). A systematic SiteAlign comparison (e) of the ATP-binding site in synapsin-I with 978 other ATP-binding sites suggest that some but not all ATP-binding sites of protein kinases (steps f, g) are similar to that of synapsin I.