Figure S1 - Comparisons of Native Shiga Toxins (Stxs) Type 1 and 2 with Chimeric Toxins Indicate that the Source of the Binding Subunit Dictates Degree of Toxicity

Stx subunits are stained in a linear manner by Oriole fluorescent stain. (A) Oriole stained SDS-Page gel of Stx1a (lanes 1–3) and Stx2a (lanes 4–6) run at equal concentrations as follows: lanes 1 and 4: 13 μg; lanes 2 and 5: 6.5 μg; lanes 3 and 6: 3.25 μg. The full A subunit is approximately 35 kDa and the cleaved A₁ subunit is approximately 32 kDa. The B subunit is approximately 7 kDa. The two-fold dilutions demonstrate a similar dose dependent fluorescent staining intensity for both Stxs. (B) Linearity of the Oriole stain. Stx concentration graphed against fluorescent intensity (arbitrary units).

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