Figure_5.tif (4.88 MB)
Comparison of transfection efficiency between PLGA/bPEI-DNA (6:3:1, w/w/w) nanoparticles and lipofectamine 2000.
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posted on 2013-09-30, 01:31 authored by Eun Jin Seo, Il Ho Jang, Eun Kyoung Do, Hyo Cheon Cheon, Soon Chul Heo, Yang Woo Kwon, Geun Ok Jeong, Ba Reun Kim, Jae Ho Kim(A) Fluorescence images of HEK293FT cells transfected with pEGFP using lipofectamine 2000 or PLGA/bPEI-DNA nanoparticles and (B) quantification of GFP signal are shown (ImageJ ver 1.43u). (C) Confocal microscopy images of HEK293FT cells co-transfected with pECFP, pEGFP, and pEYFP using lipofectamine 2000 or PLGA/bPEI-DNA nanoparticles and (D) quantification of each signal are shown (Olympus FV10-ASW ver 03.01.01.09). 1 µg of each DNA was applied to each well of a 60 mm plate. Scale bars represent 100 µm (*, p < 0.05 by Student’s t-test). n=3.
