Comparison of transfection efficiency between PLGA/bPEI-DNA (6:3:1, w/w/w) nanoparticles and lipofectamine 2000.
(A) Fluorescence images of HEK293FT cells transfected with pEGFP using lipofectamine 2000 or PLGA/bPEI-DNA nanoparticles and (B) quantification of GFP signal are shown (ImageJ ver 1.43u). (C) Confocal microscopy images of HEK293FT cells co-transfected with pECFP, pEGFP, and pEYFP using lipofectamine 2000 or PLGA/bPEI-DNA nanoparticles and (D) quantification of each signal are shown (Olympus FV10-ASW ver 03.01.01.09). 1 µg of each DNA was applied to each well of a 60 mm plate. Scale bars represent 100 µm (*, p < 0.05 by Student’s t-test). n=3.