Comparison of the ratios of sub-genomic to genome length DENV RNA in C6-36 cells infected with infectious clone-derived DENV 2 and transfected with sub-genomic DENV 2 RNA and in the accompanying culture supernate (from Figure 4).

One hundred ng of RNA from infected cells and RNA from the equivalent of 1 ul of culture supernate were used in the quantitative RT PCR reactions.